91视频 - 8MAV,电影男人鸡巴插逼,亚洲 综合 图片视频,九九中文视频播放

產(chǎn)品詳情

  • 產(chǎn)品名稱：PTH1R ELISA Kit(ParathyroidHormone1Receptor)

  • 產(chǎn)品型號：
  • 產(chǎn)品廠商：國內(nèi)供應(yīng)1
  • 產(chǎn)品文檔：
你添加了1件商品 查看購物車
簡單介紹：
PTH1R ELISA Kit(ParathyroidHormone1Receptor)
詳情介紹：
Purpose The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of PTHR1 in human tissue homogenates, cell lysates and other biological fluids.
Sample Type Biological Fluids, Cell Lysate, Tissue Homogenate
Analytical Method Quantitative
Detection Method Colorimetric
Specificity This assay has high sensitivity and excellent specificity for detection of this index.
Cross-Reactivity (Details) No significant cross-reactivity or interference between this index and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between this index and all the analogues, therefore, cross reaction may still exist.
Sensitivity 0.124 ng/mL
Components
  • Pre-coated, ready to use 96-well strip plate
  • Standard (freeze dried)
  • Standard Diluent
  • Detection Reagent A
  • Detection Reagent B
  • Assay Diluent A
  • Assay Diluent B
  • TMB
  • Stop Solution
  • Wash Buffer (30X)
  • Plate sealer for 96 wells
  • Instruction manual
Material not included
  1. Microplate reader with 450 ± 10nm filter.
  2. Precision single or multi-channel pipettes and disposable tips.
  3. Eppendorf Tubes for diluting samples.
  4. Deionized or distilled water.
  5. Absorbent paper for blotting the microtiter plate.
  6. Container for Wash Solution.
Alternative Name Parathyroid Hormone Receptor 1 (PTHR1) (PTH1R ELISA Kit Abstract)
Background Synonyms: PTH1R
Gene ID 5745
UniProt Q03431
Research Area Neurology, Endocrine system, Hormones
Pathways Regulation of Carbohydrate Metabolic Process
Sample Volume 100 μL
Assay Time 1 - 4.5 h
Plate Pre-coated
Protocol 1. Prepare all reagents, samples and standards
2. Add 100 μL standard or sample to each well. Incubate 2 hours at 37 °C
3. Aspirate and add 100 μL prepared Detection Reagent A. Incubate 1 hour at 37 °C
4. Aspirate and wash 3 times
5. Add 100 μL prepared Detection Reagent B. Incubate 30 minutes at 37 °C
6. Aspirate and wash 5 times
7. Add 90 μL Substrate Solution. Incubate 15-25 minutes at 37 °C
8. Add 50 μL Stop Solution. Read at 450nm immediately.
Assay Procedure

The microtiter plate provided in this kit has been pre-coated with an antibody specific to the index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to the index. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain the index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of the index in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Precision
  • Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
  • Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
  • CV(%) = SD/meanX100
  • Intra-assay: CV<10%
  • Inter-assay: CV<12%
Restrictions For Research Use only
Precaution of Use The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
Handling Advice The stability of ELISA Kit is determined by the loss rate of activity. The loss rate of this kit is less than 5?% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Storage 4 °C,-20 °C
Storage Comment The Assay Plate, Standard, Detection Reagent A and Detection Reagent B should be stored at -20°C upon being received. After receiving the kit , Substrate should be always stored at 4°C.Other reagents are kept according to the labels on vials. But for long term storage, please keep the whole kit at -20°C. The unused strips should be kept in a sealed bag with the desiccant provided to minimize exposure to damp air. The test kit may be used throughout the expiration date of the kit (six months from the date of manufacture). Opened test kits will remain stable until the expiring date shown, provided it is stored as prescribed above.
Expiry Date 12 months
Supplier Images
ELISA image for Parathyroid Hormone 1 Receptor (PTH1R) ELISA Kit (ABIN1572077) Parathyroid Hormone 1 Receptor (PTH1R) ELISA Kit
男人的天堂乱伦| 国产精品自拍自拍偷拍| 久久久⑨久久久| 日韩欧美国产香蕉| 日本熟人妻在线播放| 91精品欧美一区二区三区综合在| 蜜桃免费观看网站操鸡| 久久人人午夜福利| 国产99久久久免费无码| 色的久久| 日韩爽爽在线观看| 性色av一区二区三区主播| 天堂av官网| 日韩视频中文有码| 日韩七区黄片| aaa中文字幕久久久| 性生活一级毛片免费视频| 亚洲无码网站2019| 无码人妻视频在线| 亚洲一区夜| 最近欧美日韩一区二区| 好色吊香蕉久久| 天日夜夜操天天爽| 久久精品人妻,业文字幕| 一日本道a免费电影| 久久好看的国产三级片| 国产人妻精品1区2区| 国产美女精品久久久久久高潮黑人| 黑人大鸡巴狂插美女在线观看视频| heyzo.com| av亚洲黄在线| 久久中文字幕高清。| 后入重庆少妇Av电影| 男女做鸡巴视频| 欧美ab| 少妇一区二区精品人妻| 大香蕉国产一起| 性愛综合网| 麻豆免费进入1区2区3区| 精品成人3D| 久久久92午夜伦理|