91视频 - 8MAV,电影男人鸡巴插逼,亚洲 综合 图片视频,九九中文视频播放

產品詳情
  • 產品名稱:Vitronectin ELISA Kit(VTN)

  • 產品型號:
  • 產品廠商:國內供應1
  • 產品文檔:
你添加了1件商品 查看購物車
簡單介紹:
Vitronectin ELISA Kit(VTN)
詳情介紹:
Purpose The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of VTN in human serum, plasma and other biological fluids.
Sample Type Biological Fluids, Plasma, Serum
Analytical Method Quantitative
Detection Method Colorimetric
Specificity This assay has high sensitivity and excellent specificity for detection of this index.
Cross-Reactivity (Details) No significant cross-reactivity or interference between this index and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between this index and all the analogues, therefore, cross reaction may still exist.
Sensitivity 1.44 ng/mL
Components
  • Pre-coated, ready to use 96-well strip plate
  • Standard (freeze dried)
  • Standard Diluent
  • Detection Reagent A
  • Detection Reagent B
  • Assay Diluent A
  • Assay Diluent B
  • TMB
  • Stop Solution
  • Wash Buffer (30X)
  • Plate sealer for 96 wells
  • Instruction manual
Material not included
  1. Microplate reader with 450 ± 10nm filter.
  2. Precision single or multi-channel pipettes and disposable tips.
  3. Eppendorf Tubes for diluting samples.
  4. Deionized or distilled water.
  5. Absorbent paper for blotting the microtiter plate.
  6. Container for Wash Solution.
Alternative Name VTN (VTN ELISA Kit Abstract)
Background Synonyms: V75, VN, VNT, Serum Spreading Factor, Somatomedin B, Complement S-Protein
Gene ID 7448
UniProt P04004
Research Area Extracellular Matrix, Cell/Tissue Markers
Pathways Autophagy, Smooth Muscle Cell Migration
Sample Volume 100 μL
Assay Time 1 - 4.5 h
Plate Pre-coated
Protocol 1. Prepare all reagents, samples and standards
2. Add 100 μL standard or sample to each well. Incubate 2 hours at 37 °C
3. Aspirate and add 100 μL prepared Detection Reagent A. Incubate 1 hour at 37 °C
4. Aspirate and wash 3 times
5. Add 100 μL prepared Detection Reagent B. Incubate 30 minutes at 37 °C
6. Aspirate and wash 5 times
7. Add 90 μL Substrate Solution. Incubate 15-25 minutes at 37 °C
8. Add 50 μL Stop Solution. Read at 450nm immediately.
Assay Procedure

The microtiter plate provided in this kit has been pre-coated with an antibody specific to the index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to the index. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain the index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of the index in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision
  • Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
  • Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
  • CV(%) = SD/meanX100
  • Intra-assay: CV<10%
  • Inter-assay: CV<12%
Restrictions For Research Use only
Precaution of Use The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
Handling Advice The stability of ELISA Kit is determined by the loss rate of activity. The loss rate of this kit is less than 5?% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Storage 4 °C,-20 °C
Storage Comment The Assay Plate, Standard, Detection Reagent A and Detection Reagent B should be stored at -20°C upon being received. After receiving the kit , Substrate should be always stored at 4°C.Other reagents are kept according to the labels on vials. But for long term storage, please keep the whole kit at -20°C. The unused strips should be kept in a sealed bag with the desiccant provided to minimize exposure to damp air. The test kit may be used throughout the expiration date of the kit (six months from the date of manufacture). Opened test kits will remain stable until the expiring date shown, provided it is stored as prescribed above.
Expiry Date 12 months
Supplier Images
ELISA image for Vitronectin (VTN)  ELISA Kit (ABIN1573089) Vitronectin (VTN) ELISA Kit
欧美精品第十页| 91国自产精品中文字幕亚洲| 日韩精品123在线看中文字幕| 久久久日本在线观看| 欧美日韩亚洲中字一区二区三区| 黄色小说av在线| av天堂8| 女人做爱高潮不卡视频在线| 精品国产一区二区18p| 主播一区二区频道在线看| 欧美日韩色综| 北京熟妇二部| 国产一区二区三区四区 中文字幕 日韩在线播放不卡视频 | 99久久涩| 热久久麻豆国产精品| 国产一二三黄色费看| R级拍拍性视频| 久久久久久屄| 亚洲福利大片| 国产 精品 欧美 日 韩一区| 人妻 无码| 天天射亚洲精品| 亚洲高清成人综合网| 欧美专区一页| 日本中文字幕一区二区| 五月婷婷在线激情一区在线| 亚洲黄色电影网址在线观看| 亚洲精品久久99久久一区_| f国产专区欧美专区| 亚洲欧美综合图片区| 色婷婷 中文| 国标一区二区精品| 色五月麻豆免费视频在线观看,| 视频小说伦在线| 91色天天日| 日本免费电影网久久久久久| 亚洲国产99在线精品| 久久精品视频8855| 啪啪啪不卡| 亚洲大鸡巴插美女| 天天操天天爱天天爽天天干|