91视频 - 8MAV,电影男人鸡巴插逼,亚洲 综合 图片视频,九九中文视频播放

產(chǎn)品詳情

  • 產(chǎn)品名稱：MMP2 ELISA Kit(MatrixMetalloproteinase2)

  • 產(chǎn)品型號(hào)：
  • 產(chǎn)品廠商：國內(nèi)供應(yīng)1
  • 產(chǎn)品文檔：
你添加了1件商品 查看購物車
簡單介紹：
MMP2 ELISA Kit(MatrixMetalloproteinase2)
詳情介紹：
Purpose The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of MMP2 in mouse serum, plasma, tissue homogenates and other biological fluids.
Sample Type Biological Fluids, Plasma, Serum, Tissue Homogenate
Analytical Method Quantitative
Detection Method Colorimetric
Specificity This assay has high sensitivity and excellent specificity for detection of this index.
Cross-Reactivity (Details) No significant cross-reactivity or interference between this index and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between this index and all the analogues, therefore, cross reaction may still exist.
Sensitivity 0.065 ng/mL
Components
  • Pre-coated, ready to use 96-well strip plate
  • Standard (freeze dried)
  • Standard Diluent
  • Detection Reagent A
  • Detection Reagent B
  • Assay Diluent A
  • Assay Diluent B
  • TMB
  • Stop Solution
  • Wash Buffer (30X)
  • Plate sealer for 96 wells
  • Instruction manual
Material not included
  1. Microplate reader with 450 ± 10nm filter.
  2. Precision single or multi-channel pipettes and disposable tips.
  3. Eppendorf Tubes for diluting samples.
  4. Deionized or distilled water.
  5. Absorbent paper for blotting the microtiter plate.
  6. Container for Wash Solution.
Alternative Name MMP2 (MMP2 ELISA Kit Abstract)
Background Synonyms: CLG4, CLG4A, MMP-II, MONA, TBE-1, Gelatinase A, 72kDa Gelatinase, 72kDa Type IV Collagenase
Gene ID 17390
UniProt P33434
Research Area Extracellular Matrix
Pathways Activation of Innate immune Response
Sample Volume 100 μL
Assay Time 1 - 4.5 h
Plate Pre-coated
Protocol 1. Prepare all reagents, samples and standards
2. Add 100 μL standard or sample to each well. Incubate 2 hours at 37 °C
3. Aspirate and add 100 μL prepared Detection Reagent A. Incubate 1 hour at 37 °C
4. Aspirate and wash 3 times
5. Add 100 μL prepared Detection Reagent B. Incubate 30 minutes at 37 °C
6. Aspirate and wash 5 times
7. Add 90 μL Substrate Solution. Incubate 15-25 minutes at 37 °C
8. Add 50 μL Stop Solution. Read at 450nm immediately.
Assay Procedure

The microtiter plate provided in this kit has been pre-coated with an antibody specific to the index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to the index. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain the index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of the index in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Precision
  • Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
  • Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
  • CV(%) = SD/meanX100
  • Intra-assay: CV<10%
  • Inter-assay: CV<12%
Restrictions For Research Use only
Precaution of Use The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
Handling Advice The stability of ELISA Kit is determined by the loss rate of activity. The loss rate of this kit is less than 5?% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Storage 4 °C,-20 °C
Storage Comment The Assay Plate, Standard, Detection Reagent A and Detection Reagent B should be stored at -20°C upon being received. After receiving the kit , Substrate should be always stored at 4°C.Other reagents are kept according to the labels on vials. But for long term storage, please keep the whole kit at -20°C. The unused strips should be kept in a sealed bag with the desiccant provided to minimize exposure to damp air. The test kit may be used throughout the expiration date of the kit (six months from the date of manufacture). Opened test kits will remain stable until the expiring date shown, provided it is stored as prescribed above.
Expiry Date 12 months
Supplier Images
ELISA image for Matrix Metalloproteinase 2 (MMP2) ELISA Kit (ABIN1029244) Matrix Metalloproteinase 2 (MMP2) ELISA Kit
深爱五月激情,| 成人亚洲中文在线| 国产 做受视频| 日韩国产欧美第一页| 欧美无专区| Japan一区二区video| 最新大香蕉在线视频| 读书| 欧美A A A一级| 狠狠88色狠狠色综合久久人天| 3D艹B在线观看| 欧美不卡在线一区二区| 91久久久久精| 欧美幼一区二区| 欧美3d一区二区三区四区| 后入韩国三级在线视频| 久久99热国产| 日本一二三色综合| 日本亚洲欧成人| 一本久道天天日天天干| 欧美 日韩 一区 图| 色偷偷人人澡久久超碰91蜜臀| 欧美在线一区播放| 亚洲欧美激情小说另类| 久久综合中文字幕色| 国产精品第九十七页| 国产,欧美在线综合| 一区二区精品VA| 亚洲热欧美| 蜜桃AV人体| 欧美黑料泄密中文无码一区二区| 大香蕉在线伊| 五月激情麻豆| 午夜无码在线观看| 欧美精品一区二区在线观看福利| 天天射天天操免费视频| 久久依人大香蕉| 精品欧美精品| 亚洲视频1区2区| 色综合久久悠悠| 在线看黄AV免费看|