91视频 - 8MAV,电影男人鸡巴插逼,亚洲 综合 图片视频,九九中文视频播放

產(chǎn)品詳情

  • 產(chǎn)品名稱：IgA ELISA Kit

  • 產(chǎn)品型號(hào)：
  • 產(chǎn)品廠商：國(guó)內(nèi)供應(yīng)1
  • 產(chǎn)品文檔：
你添加了1件商品 查看購(gòu)物車
簡(jiǎn)單介紹：
IgA ELISA Kit
詳情介紹：
Purpose The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of IgA in rat serum, plasma and other biological fluids.
Sample Type Biological Fluids, Plasma, Serum
Analytical Method Quantitative
Detection Method Colorimetric
Specificity This assay has high sensitivity and excellent specificity for detection of this index.
Cross-Reactivity (Details) No significant cross-reactivity or interference between this index and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between this index and all the analogues, therefore, cross reaction may still exist.
Sensitivity 0.37 ng/mL
Components
  • Pre-coated, ready to use 96-well strip plate
  • Standard (freeze dried)
  • Standard Diluent
  • Detection Reagent A
  • Detection Reagent B
  • Assay Diluent A
  • Assay Diluent B
  • TMB
  • Stop Solution
  • Wash Buffer (30X)
  • Plate sealer for 96 wells
  • Instruction manual
Material not included
  1. Microplate reader with 450 ± 10nm filter.
  2. Precision single or multi-channel pipettes and disposable tips.
  3. Eppendorf Tubes for diluting samples.
  4. Deionized or distilled water.
  5. Absorbent paper for blotting the microtiter plate.
  6. Container for Wash Solution.
Sample Volume 100 μL
Assay Time 1 - 4.5 h
Plate Pre-coated
Protocol 1. Prepare all reagents, samples and standards
2. Add 100 μL standard or sample to each well. Incubate 2 hours at 37 °C
3. Aspirate and add 100 μL prepared Detection Reagent A. Incubate 1 hour at 37 °C
4. Aspirate and wash 3 times
5. Add 100 μL prepared Detection Reagent B. Incubate 30 minutes at 37 °C
6. Aspirate and wash 5 times
7. Add 90 μL Substrate Solution. Incubate 15-25 minutes at 37 °C
8. Add 50 μL Stop Solution. Read at 450nm immediately.
Assay Procedure

The microtiter plate provided in this kit has been pre-coated with an antibody specific to the index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to the index. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain the index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of the index in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Precision
  • Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
  • Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
  • CV(%) = SD/meanX100
  • Intra-assay: CV<10%
  • Inter-assay: CV<12%
Restrictions For Research Use only
Precaution of Use The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
Handling Advice The stability of ELISA Kit is determined by the loss rate of activity. The loss rate of this kit is less than 5?% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Storage 4 °C,-20 °C
Storage Comment The Assay Plate, Standard, Detection Reagent A and Detection Reagent B should be stored at -20°C upon being received. After receiving the kit , Substrate should be always stored at 4°C.Other reagents are kept according to the labels on vials. But for long term storage, please keep the whole kit at -20°C. The unused strips should be kept in a sealed bag with the desiccant provided to minimize exposure to damp air. The test kit may be used throughout the expiration date of the kit (six months from the date of manufacture). Opened test kits will remain stable until the expiring date shown, provided it is stored as prescribed above.
Expiry Date 12 months
午夜三级伦理国产| 99热18精品| 中文字暮Av一区二区三区| 日本色道久久一区二区| 久久超碰乱码在线| 国产 欧美 日韩 在线 极品 | 日韩三级人妇| 香蕉视频黄色一级免费| 美女高潮视频网址| 97麻豆成人网| 亚洲天堂欧美成人| 欧美黄色网| AV美女在线亚洲| av黄色在线看不卡的免费的| 日日操夜夜操免费观看视频| 非洲1级黄片| 久久22| 69m.国产福利在线| 精品国产一区不卡高清| 亚洲黄色成人视频久久久| 久久sb综合| 亚洲美女裸体香蕉视频精品| se久久久久久| 日韩专区 欧美 一区二区| 黄色夫妻视频免费观看| 内射日韩美女人一| 久久亚洲最新地址一| 日韩在线性生活视频| 国产欧美色1区2区| 日B片高清视频在线观看| 日韩欧美第15页| 久久 AV电影| 成人乱码偷拍一区二区| 开心熟妇网| AV婷婷综合五月| 日本夕夕久久久久久久久| 久久久久国产精品麻豆影视| 精品一区二区三区69| 黄片影院免费观看| 久久久久亚洲三级片久久久久| 国精产品久久一区二区|