91视频 - 8MAV,电影男人鸡巴插逼,亚洲 综合 图片视频,九九中文视频播放

產品詳情

  • 產品名稱：C5A ELISA Kit(ComplementComponent5a)

  • 產品型號：
  • 產品廠商：國內供應1
  • 產品文檔：
你添加了1件商品 查看購物車
簡單介紹：
C5A ELISA Kit(ComplementComponent5a)
詳情介紹：
Purpose The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of C5a in rabbit serum, plasma and other biological fluids.
Sample Type Biological Fluids, Plasma, Serum
Analytical Method Quantitative
Detection Method Colorimetric
Specificity This assay has high sensitivity and excellent specificity for detection of this index.
Cross-Reactivity (Details) No significant cross-reactivity or interference between this index and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between this index and all the analogues, therefore, cross reaction may still exist.
Sensitivity 2.86 pg/mL
Components
  • Pre-coated, ready to use 96-well strip plate
  • Standard (freeze dried)
  • Standard Diluent
  • Detection Reagent A
  • Detection Reagent B
  • Assay Diluent A
  • Assay Diluent B
  • TMB
  • Stop Solution
  • Wash Buffer (30X)
  • Plate sealer for 96 wells
  • Instruction manual
Material not included
  1. Microplate reader with 450 ± 10nm filter.
  2. Precision single or multi-channel pipettes and disposable tips.
  3. Eppendorf Tubes for diluting samples.
  4. Deionized or distilled water.
  5. Absorbent paper for blotting the microtiter plate.
  6. Container for Wash Solution.
Alternative Name C5a (C5a ELISA Kit Abstract)
Pathways Complement System, Carbohydrate Homeostasis
Sample Volume 100 μL
Assay Time 1 - 4.5 h
Plate Pre-coated
Protocol 1. Prepare all reagents, samples and standards
2. Add 100 μL standard or sample to each well. Incubate 2 hours at 37 °C
3. Aspirate and add 100 μL prepared Detection Reagent A. Incubate 1 hour at 37 °C
4. Aspirate and wash 3 times
5. Add 100 μL prepared Detection Reagent B. Incubate 30 minutes at 37 °C
6. Aspirate and wash 5 times
7. Add 90 μL Substrate Solution. Incubate 15-25 minutes at 37 °C
8. Add 50 μL Stop Solution. Read at 450nm immediately.
Assay Procedure

The microtiter plate provided in this kit has been pre-coated with an antibody specific to the index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to the index. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain the index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of the index in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Precision
  • Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
  • Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
  • CV(%) = SD/meanX100
  • Intra-assay: CV<10%
  • Inter-assay: CV<12%
Restrictions For Research Use only
Precaution of Use The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
Handling Advice The stability of ELISA Kit is determined by the loss rate of activity. The loss rate of this kit is less than 5?% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Storage 4 °C,-20 °C
Storage Comment The Assay Plate, Standard, Detection Reagent A and Detection Reagent B should be stored at -20°C upon being received. After receiving the kit , Substrate should be always stored at 4°C.Other reagents are kept according to the labels on vials. But for long term storage, please keep the whole kit at -20°C. The unused strips should be kept in a sealed bag with the desiccant provided to minimize exposure to damp air. The test kit may be used throughout the expiration date of the kit (six months from the date of manufacture). Opened test kits will remain stable until the expiring date shown, provided it is stored as prescribed above.
Expiry Date 12 months
欧美丝袜色图在线| 免费精品视频一区| 免费看日本黄色大片| 污在线观看| 日本熟女中文在线| 在线观看无码,| 区二区三区在线| 午夜福利二| 日本不卡视颜| z中文字幕在线观看| 欧美,日韩,亚洲,色欲| 麻豆黄色 插插视频| 一区二区三区毛片大全| 久久精品丰满少妇免弗| 欧美大片特级特黄的免费的| av日韩国产在线香蕉| 男男大鸡巴操大鸡巴网站| 久久精品女人天堂AV| 少妇性久久久久| 欧美日韩大胆一级视频| “国产一区”| 欧美国产一二三| 天天日天天干APP| 熟妇人妻久久久| 在线观看福利小视频| 久久久久久久免费精品视频| 久久精品又黄又w网站| 91久久麻豆欧美精品| 欧美大鸡巴操亚洲人| 国产美女舔弄我的鸡巴av| 伊人操逼222| 欧美专利1去2区2区5区免费| 在线免费观看久艹| 国产三级成人网站| 艹骚逼动漫视频| 人人操人人舔老熟女| 黑人双叉欧美| 99国产精品久久久久电影| 大鸡吧操逼美女的大屁股真舒服| 欧美片轻轻插久久久| 日韩人妻丝袜在线|