91视频 - 8MAV,电影男人鸡巴插逼,亚洲 综合 图片视频,九九中文视频播放

產(chǎn)品詳情

  • 產(chǎn)品名稱：VWF ELISA Kit(VonWillebrandFactor)

  • 產(chǎn)品型號：
  • 產(chǎn)品廠商：國內(nèi)供應(yīng)1
  • 產(chǎn)品文檔：
你添加了1件商品 查看購物車
簡單介紹：
VWF ELISA Kit(VonWillebrandFactor)
詳情介紹：
Purpose ,The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of vWF in human plasma and other biological fluids.
Sample Type Biological Fluids, Plasma
Analytical Method Quantitative
Detection Method Colorimetric
Specificity This assay has high sensitivity and excellent specificity for detection of this index.
Cross-Reactivity (Details) No significant cross-reactivity or interference between this index and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between this index and all the analogues, therefore, cross reaction may still exist.
Sensitivity 0.53 ng/mL
Components
  • Pre-coated, ready to use 96-well strip plate
  • Standard (freeze dried)
  • Standard Diluent
  • Detection Reagent A
  • Detection Reagent B
  • Assay Diluent A
  • Assay Diluent B
  • TMB
  • Stop Solution
  • Wash Buffer (30X)
  • Plate sealer for 96 wells
  • Instruction manual
Material not included
  1. Microplate reader with 450 ± 10nm filter.
  2. Precision single or multi-channel pipettes and disposable tips.
  3. Eppendorf Tubes for diluting samples.
  4. Deionized or distilled water.
  5. Absorbent paper for blotting the microtiter plate.
  6. Container for Wash Solution.
Alternative Name vWF (VWF ELISA Kit Abstract)
Background Synonyms: F8VWF, VWD
Gene ID 7450
UniProt P04275
Research Area Coagulation
Sample Volume 100 μL
Assay Time 1 - 4.5 h
Plate Pre-coated
Protocol 1. Prepare all reagents, samples and standards
2. Add 100 μL standard or sample to each well. Incubate 2 hours at 37 °C
3. Aspirate and add 100 μL prepared Detection Reagent A. Incubate 1 hour at 37 °C
4. Aspirate and wash 3 times
5. Add 100 μL prepared Detection Reagent B. Incubate 30 minutes at 37 °C
6. Aspirate and wash 5 times
7. Add 90 μL Substrate Solution. Incubate 15-25 minutes at 37 °C
8. Add 50 μL Stop Solution. Read at 450nm immediately.
Assay Procedure

The microtiter plate provided in this kit has been pre-coated with an antibody specific to the index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to the index. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain the index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of the index in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Precision
  • Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
  • Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
  • CV(%) = SD/meanX100
  • Intra-assay: CV<10%
  • Inter-assay: CV<12%
Restrictions For Research Use only
Precaution of Use The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
Handling Advice The stability of ELISA Kit is determined by the loss rate of activity. The loss rate of this kit is less than 5?% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Storage 4 °C,-20 °C
Storage Comment The Assay Plate, Standard, Detection Reagent A and Detection Reagent B should be stored at -20°C upon being received. After receiving the kit , Substrate should be always stored at 4°C.Other reagents are kept according to the labels on vials. But for long term storage, please keep the whole kit at -20°C. The unused strips should be kept in a sealed bag with the desiccant provided to minimize exposure to damp air. The test kit may be used throughout the expiration date of the kit (six months from the date of manufacture). Opened test kits will remain stable until the expiring date shown, provided it is stored as prescribed above.
Expiry Date 12 months
Supplier Images
ELISA image for Von Willebrand Factor (VWF) ELISA Kit (ABIN1030190) Von Willebrand Factor (VWF) ELISA Kit
成人网站在线爽爽爽| 一区二区高清无码在线| 91在线精品秘 一区二区九色| 懂色无码精品一区二区| av呦呦| 美女高潮www| 日韩的一区| 欧美日韩一级无码| 东北色婷婷综合在线| 青青草久久婷婷五月| 欧美日韩一道在线_亚洲欧美日韩中| 亚洲无码欧毛| 青娱乐免费视频一区二区| 黄色亚洲影院| 就是干av在线| 国产成人免费一级A| 日本AAA高清视频一区二区| 日韩网站色| 亚欧日韩欧美网站在线看| 日韩国产五月天婷婷在线观看| 大屌干第二区| 婷婷在线播放| 人妻在线理论影院| 人妻无码3区| 日本熟女久久| 一区二区三区欧美在线| 猛进猛出日本人妻| 久久人主妻一区二区| 夫夫妻性视频1区2区3区| 亚洲黄色毛片,一区二区| 丁香五月婷婷在线动漫| 国产欧美精神| 少妇oav| 精品人妻无码视频中文字幕一| av激情在线高清| 大香蕉视频在线观看视频在线| 国产超碰在线女人味| 久久奸少妇| 97色午夜| 五月婷不卡视频二区| 日韩91欧美999|