91视频 - 8MAV,电影男人鸡巴插逼,亚洲 综合 图片视频,九九中文视频播放

產品詳情

  • 產品名稱：Kininogen1(KNG1) ELISA Kit

  • 產品型號：
  • 產品廠商：國內供應1
  • 產品文檔：
你添加了1件商品 查看購物車
簡單介紹：
Kininogen1(KNG1) ELISA Kit
詳情介紹：
Purpose The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of BK in rat serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Sample Type Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids
Analytical Method Quantitative
Detection Method Colorimetric
Specificity This assay has high sensitivity and excellent specificity for detection of this index.
Cross-Reactivity (Details) No significant cross-reactivity or interference between this index and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between this index and all the analogues, therefore, cross reaction may still exist.
Sensitivity 8.53 pg/mL
Components
  • Pre-coated, ready to use 96-well strip plate
  • Standard (freeze dried)
  • Standard Diluent
  • Detection Reagent A
  • Detection Reagent B
  • Assay Diluent A
  • Assay Diluent B
  • TMB
  • Stop Solution
  • Wash Buffer (30X)
  • Plate sealer for 96 wells
  • Instruction manual
Material not included
  1. Microplate reader with 450 ± 10nm filter.
  2. Precision single or multi-channel pipettes and disposable tips.
  3. Eppendorf Tubes for diluting samples.
  4. Deionized or distilled water.
  5. Absorbent paper for blotting the microtiter plate.
  6. Container for Wash Solution.
Alternative Name Bradykinin (BK) (KNG1 ELISA Kit Abstract)
Pathways ACE Inhibitor Pathway, Glycosaminoglycan Metabolic Process
Sample Volume 100 μL
Assay Time 1 - 4.5 h
Plate Pre-coated
Protocol 1. Prepare all reagents, samples and standards
2. Add 100 μL standard or sample to each well. Incubate 2 hours at 37 °C
3. Aspirate and add 100 μL prepared Detection Reagent A. Incubate 1 hour at 37 °C
4. Aspirate and wash 3 times
5. Add 100 μL prepared Detection Reagent B. Incubate 30 minutes at 37 °C
6. Aspirate and wash 5 times
7. Add 90 μL Substrate Solution. Incubate 15-25 minutes at 37 °C
8. Add 50 μL Stop Solution. Read at 450nm immediately.
Assay Procedure

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to the index has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled the index and unlabeled the index (Standards or samples) with the pre-coated antibody specific to the index. After incubation the unbound conjugate is washed off. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of the index in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of the index in the sample.
Assay Precision
  • Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
  • Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
  • CV(%) = SD/meanX100
  • Intra-assay: CV<10%
  • Inter-assay: CV<12%
Restrictions For Research Use only
Precaution of Use The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
Handling Advice The stability of ELISA Kit is determined by the loss rate of activity. The loss rate of this kit is less than 5?% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Storage 4 °C,-20 °C
Storage Comment The Assay Plate, Standard, Detection Reagent A and Detection Reagent B should be stored at -20°C upon being received. After receiving the kit , Substrate should be always stored at 4°C.Other reagents are kept according to the labels on vials. But for long term storage, please keep the whole kit at -20°C. The unused strips should be kept in a sealed bag with the desiccant provided to minimize exposure to damp air. The test kit may be used throughout the expiration date of the kit (six months from the date of manufacture). Opened test kits will remain stable until the expiring date shown, provided it is stored as prescribed above.
Expiry Date 12 months
欧美精品超清在线| 超碰人妻天天摸天天操| 欧美一区在线综合都市激情| 免费国产wwwwwww网站| Japanese69无码一区| 久久成人拍拍| a美女视频久久| 园产精品福利AV| 亚洲在线黄片| av怡红院| 欧美超黄片| 社会| 手机无码综合在线| 五码一区二区以| 精品一区二区三区AV美女| 激情老熟女合久丁| 婷婷字幕网| 国产精品无码9999| 久久久久久久久久久久婷| 日本熟久久久久久久久| 老熟女精品久久| 国产精品久久久久久久漫画软件| 亚洲欧洲无码一区二区三区| 涩涩网站在线观看| 天天日日人人夜夜狠狠干| 日韩欧美丝袜另类人妻| 老司机中文字幕无码网站| 男女又粗又长又大又黄视频 | 日本黄色麻豆网站免费在线看片| 欧美成人性生活久久久久久久| 国产亚洲欧美中文在线BT天堂| 亚洲AV无码变态另类专区水| 超碰人妻免费视屏| 欧美日韩区一| 欧美日韩女性3P| 欧美在线观看黄| 熟女大香蕉视频狼性| 欧美天天干免费视频| 乱码一区二区欧美| 综合久久不卡| 亚洲日韩另类欧美精品久久|