91视频 - 8MAV,电影男人鸡巴插逼,亚洲 综合 图片视频,九九中文视频播放

產(chǎn)品詳情

  • 產(chǎn)品名稱：Dihydrotestosterone(DHT) ELISA Kit

  • 產(chǎn)品型號(hào)：
  • 產(chǎn)品廠商：國(guó)內(nèi)供應(yīng)1
  • 產(chǎn)品文檔：
你添加了1件商品 查看購(gòu)物車
簡(jiǎn)單介紹：
Dihydrotestosterone(DHT) ELISA Kit
詳情介紹：
Purpose The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of DHT in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Sample Type Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids
Analytical Method Quantitative
Detection Method Colorimetric
Specificity This assay has high sensitivity and excellent specificity for detection of this index.
Cross-Reactivity (Details) No significant cross-reactivity or interference between this index and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between this index and all the analogues, therefore, cross reaction may still exist.
Sensitivity 16.15 pg/mL
Components
  • Pre-coated, ready to use 96-well strip plate
  • Standard (freeze dried)
  • Standard Diluent
  • Detection Reagent A
  • Detection Reagent B
  • Assay Diluent A
  • Assay Diluent B
  • TMB
  • Stop Solution
  • Wash Buffer (30X)
  • Plate sealer for 96 wells
  • Instruction manual
Material not included
  1. Microplate reader with 450 ± 10nm filter.
  2. Precision single or multi-channel pipettes and disposable tips.
  3. Eppendorf Tubes for diluting samples.
  4. Deionized or distilled water.
  5. Absorbent paper for blotting the microtiter plate.
  6. Container for Wash Solution.
Sample Volume 100 μL
Assay Time 1 - 4.5 h
Plate Pre-coated
Protocol 1. Prepare all reagents, samples and standards
2. Add 50 μL standard or sample to each well. And then add 50 μL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37 °C
3. Aspirate and wash 3 times
4. Add 100?L prepared Detection Reagent B. Incubate 30 minutes at 37 °C
5. Aspirate and wash 5 times
6. Add 90?L Substrate Solution. Incubate 15-25 minutes at 37 °C
7. Add 50?L Stop Solution. Read at 450nm immediately.
Assay Procedure

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to the index has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled the index and unlabeled the index (Standards or samples) with the pre-coated antibody specific to the index. After incubation the unbound conjugate is washed off. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of the index in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of the index in the sample.
Assay Precision
  • Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
  • Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
  • CV(%) = SD/meanX100
  • Intra-assay: CV<10%
  • Inter-assay: CV<12%
Restrictions For Research Use only
Precaution of Use The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
Handling Advice The stability of ELISA Kit is determined by the loss rate of activity. The loss rate of this kit is less than 5?% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Storage 4 °C,-20 °C
Storage Comment The Assay Plate, Standard, Detection Reagent A and Detection Reagent B should be stored at -20°C upon being received. After receiving the kit , Substrate should be always stored at 4°C.Other reagents are kept according to the labels on vials. But for long term storage, please keep the whole kit at -20°C. The unused strips should be kept in a sealed bag with the desiccant provided to minimize exposure to damp air. The test kit may be used throughout the expiration date of the kit (six months from the date of manufacture). Opened test kits will remain stable until the expiring date shown, provided it is stored as prescribed above.
Expiry Date 12 months
美区一区二区| 色婷婷综合久久久久久中文字幕| 超碰色欲一区| 精彩无码一区两区| 日韩理伦片在线网站| 美女日逼视频污污| 色大师熟女一区二时期| 一区二网区网站| 欧日韩在线观看免费精品| 亚洲精品一区二区午夜福利| 久久思欧美人| 超碰大AV| 国产夫妻性生活在视频播放| 色综合精品免费| 久久久久久久久久98精品| 日本不卡的三区四去污区| 久久一区二区三区精品| 久久国产精品免费一区| 欧美色图片婷婷| 亚洲大尺度在线观看高清视频| AV无码免费久久久精品| 人人妻人人超人人喊| 日韩有码一区牛牛| 欧美淫荡一二三区| 极品尤物一区| 国产内射电影自拍| 亚洲中出无码一区二区| 俄罗斯男女嬲别视频| 国产精品小h片一区二区| 老鸭窝午夜成人视频| 黑人双叉欧美| 日韩日b直接看| 91小仙女思妍是谁| 高清av无码不卡| 无码国产79精品久久久久| 综合网人妻| 小黄欧美一级视频| 日本三级片在线观看一区二区三道| 国产女人喷水在线观看| 男生福利网站| 日大香蕉视频|